纯素食阿多尼斯不平衡设计SS类型II或III [英] Vegan adonis unbalanced design SS type II or III

问题描述

我是多元统计的新手，所以如果这个问题很天真，或者我错过了重要的事情，请原谅我.

I am a newbie to multivariate statistics, so please forgive me if this question is naïve, or if I have missed something important.

我想知道在使用阿多尼斯时如何处理不平衡的设计.我有一个数据集，该数据集包含河流沉积物中的微生物群落，以及水流(4个水平)，US_DS(2个水平)和季节(2个水平)(我也有其他因素，但目前仍坚持这些因素).例如下面的代码:

I'd like to know how to deal with an unbalanced design when using adonis. I have a data set of microbial communities present in stream sediment and the factors stream (4 levels), US_DS (2 levels) and season (2 levels) (I have other factors as well but sticking to these for the moment). e.g. code below:

dist.L2<-vegdist(L2, mehod="bray")
adonis(dist.L2~Season*Stream*US_DS, data=factors, permutations=199)

不幸的是，设计不平衡(某些站点只有1个副本，而其他站点最多有3个).

Unfortunately the design is unbalanced (some sites only have 1 replicate, while others have up to 3).

我曾经尝试过运行adonis，但是因为anodis使用1型SS，所以我将因素放入的顺序有很大的不同，因此没有理由将一个因素放在其他因素之前.我知道其他统计数据包都可以通过使用II型或III型平方和来解决此问题，并且无论您使用哪种类型的SS都有优点和缺点.我的数据的性质意味着其他更简单的统计数据测试实际上并不适合.

I have tried running adonis, however because anodis uses type 1 SS the order that I put the factors in makes a large difference, and there is no reason to put one factor before the others. I know that other stats packages get around this by using type II or III sums of squares, and that there are advantages and disadvantages no matter what type of SS you use. The nature of my data means that other more simple stats tests are not really appropriate.

我想知道如何将III型错误与Adonis一起使用，或者是否有找到I型错误和Adonis的程序来寻找合适的测试.谢谢

I would like to know how to either use type III errors with adonis, or whether there is a procedure to go through using type I errors and adonis as it is to find an appropriate test. Thanks

推荐答案

您可能需要通过更改两个因素的顺序来做两次Adonis.

You may need to do Adonis twice by changing the order of your two factors.

edit 先前提供的参考链接已删除，因为不再有效

edit the previously supplied reference link was removed because not active any more

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