snakemake中的未知输出 [英] unknown output in snakemake
问题描述
我正在用snakemake实现一个非常简单的管道,希望用一个有凝聚力的Snakefile代替一连串烦人的bash脚本.
I'm working on implementing a very simple pipeline in snakemake in hopes of replacing a chain of annoying bash scripts with one cohesive Snakefile.
我在编写将文件分割成较小的规则(使用GNU split),然后导致输出连接在一起的第二条规则时遇到了麻烦.
I'm having trouble writing a rule that splits a file into smaller pieces (using GNU split), and then leads to a second rule where the output is concatenated together.
我不知道在concat步骤中为输入内容写什么,因为我不知道如何定义所有符合模式 bam_files/test *
的文件.我尝试使用glob,但是绝对不能正常工作(似乎实际上是在跳过包含glob的拆分).有什么更好的办法可以做到这一点吗?
I don't know what to write for the input in the concat step, since I don't know how to define all the files fitting the pattern bam_files/test*
. I tried with glob, but that decidedly doesn't seem to work (it seems like it's actually skipping split altogether with the glob included). Is there any better way that I could be doing this?
# test snakemake pipeline
import glob
SAMPLE_IDS = ["test"]
rule all:
input:
expand("bam_files/{FASTQ}.out", FASTQ=SAMPLE_IDS)
rule split:
input:
expand("{FASTQ}.txt", FASTQ=SAMPLE_IDS)
output:
"bam_files/{FASTQ}."
shell:
"cat {input} | split -l 1000 -d - {output}."
rule concat:
input:
split_files = glob.glob("bam_files/{FASTQ}.*")
output:
"bam_files/{FASTQ}.out"
shell:
"cat {input} > {output}"
推荐答案
我认为这应该有效:
SAMPLE_IDS = ["test"]
rule all:
input:
expand("bam_files/{FASTQ}.out", FASTQ=SAMPLE_IDS)
rule split:
input:
"{FASTQ}.txt"
output:
dynamic("bam_files/{FASTQ}.{PART}")
params:
length=1000
shell:
"cat {input} | split -l {params.length} -d - bam_files/{FASTQ}."
rule concat:
input:
split_files = dynamic("bam_files/{FASTQ}.{PART}")
output:
"bam_files/{FASTQ}.out"
shell:
"cat {input} > {output}"
看来 split
规则应该一次获取一个文件 {FASTQ} .txt
并生成 {FASTQ} .1,{FASTQ}.2,...
或类似的内容.因为您不预先知道它将产生多少个文件,所以您需要对 split.output
和 concat.input <都使用
dynamic()
/code>.
It looks like the split
rule should be taking one file {FASTQ}.txt
at a time and producing {FASTQ}.1, {FASTQ}.2, ...
or something similar. Because you don't know ahead of time how many files it will produce, you need to use dynamic()
for both split.output
and concat.input
.
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